dark-field(常见于 dark-field microscopy)指暗场(成像/照明):一种显微观察方法,通过让背景保持黑暗、只让样品散射的光进入物镜,使透明或细小结构呈现明亮轮廓,从而提高对比度。
/ˈdɑːrk fiːld/
Dark-field microscopy makes tiny particles easier to see.
暗场显微镜能让微小颗粒更容易被看见。
Under dark-field illumination, the transparent bacteria appeared as bright shapes against a black background, revealing details that were hard to detect in bright-field images.
在暗场照明下,透明细菌在黑色背景上呈现明亮形状,显现出在明场图像中难以察觉的细节。
由 dark(黑暗的)+ field(视野、场)组成,字面意思是“黑暗的视场”。该术语主要在光学与显微技术中使用,强调成像时背景被压暗、目标因散射光而“亮起来”的效果。